Fluorescence enzyme immunoassay for insulin using peroxidase-tyramine-hydrogen peroxide.

Fluorescence determination of hydrogen peroxide using hemoglobin as a mimetic enzyme of peroxidase.

Flow-through micro sensor using immobilized peroxidase with chemiluminometric FIA system for determining hydrogen peroxide.

A micromachined flow cell (overall size; 25 x 25 x 1 mm3) was designed for the fast determination of hydrogen peroxide, based on a luminol-H2O2 chemiluminescence reaction catalyzed by immobilized peroxidase (POD). The flow cell consisted of a sandwich of anisotropically etched silicon and glass chips and contained a spiral channel (20 turns, 50 cm long, 150 microm wide, 20 microm depth, channel...

Modeling the reduction of hydrogen peroxide by glutathione peroxidase mimics.

Theoretical calculations have been performed on three model reactions representing the reduction of hydrogen peroxide by ebselen, ebselen selenol, and ebselen diselenide. The reaction surfaces have been investigated at the B3PW91/6-311G(2df,p) level, and single-point energies were calculated using the 6-311++G(3df,3pd) basis set. Solvent effects were included implicitly with the conductor-like ...

Reaction of Thiol Compounds with Peroxidase and Hydrogen Peroxide*

Recent studies have indicated that peroxidase might function in the biological synthesis of thyroxine by the thyroid gland. Johnson and Tewkesbury (1) believed that thyroxine might be formed by the oxidative coupling of 2 molecules of diiodotyrosine. Westerfeld and Lowe (2) studied the oxidative condensation of p-cresol by hydrogen peroxide and peroxidase and suggested that peroxidase might be ...

The reaction of ferrous horseradish peroxidase with hydrogen peroxide.

Hydrogen peroxide reacts with ferrous horseradish peroxidase and converts it to oxyperoxidase in a sequence of two reactions. The first is the reaction of ferrous peroxidase with HzOz to form Compound II; the second is the reaction of Compound II with HzOz to form oxyperoxidase. Both reactions follow second order kinetics, being first order with respect to each of the reactants. They proceed wi...